Hybridization Chain Reaction

We develop a new cell targeting fluorescent probe that enables rna imaging in living mice via an in vivo hybridization chain reaction hcr.

Hybridization chain reaction. Hybridization chain reaction hcr is a simple isothermal enzyme free process with the advantages of high simplicity and versatility. A new technique based on a chain reaction of recognition and hybridization events between two sets of dna hairpin molecules offers an enzyme free alternative for the rapid detection of specific. Rna imaging in living animals helps decipher biology and creates new theranostics for disease treatment. In the simplest version of this process two stable species of dna hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers.

A novel method for controlling the oligomerization of metastable dna hairpins using the hybridization chain reaction hcr is reported. Control was achieved through the introduction of a base pair mismatch in the duplex of the hairpins. Bi s 1 yue s zhang s. Generally initiator single stranded dna is amplified to form long double stranded dna based on a chain reaction during which recognition and hybridization events between a pair of complementary dna sequences occur in succession.

The mismatch modification allows one to kinetically differentiate initiation versus propagation events leading to dna oligomers up to 10 monomers long and. We introduce the concept of hybridization chain reaction hcr in which stable dna monomers assemble only upon exposure to a target dna fragment. In situ hybridization based on the mechanism of the hybridization chain reaction hcr has addressed multi decade challenges that impeded imaging of mrna expression in diverse organisms offering a unique combination of multiplexing quantitation sensitivity resolution and versatility. In particular hybridization chain reaction hcr a type of toehold mediated strand displacement tmsd reaction has attracted great interest because of its enzyme free nature isothermal conditions simple protocols and excellent amplification efficiency.

1 collaborative innovation center for marine biomass fiber materials and textiles of shandong province college of chemistry and chemical engineering shandong sino japanese center. Pyrene excimer probes based on the hybridization chain reaction for the detection of nucleic acids in complex biological fluids jin huang state key laboratory of chemo biosensing and chemometrics college of biology and college of chemistry and chemical engineering hunan university changsha 410082 p r. Herein we present an ultrasensitive concatenated hybridization chain reaction c hcr based surface enhanced raman scattering sers immunoassay by forming antibody antigen aptamer heterosandwich structures with the model analyte of total prostate.